Questions and Answers on DNA and MRNA

Questions and Answers on DNA and MRNA Rumana Anam Q1. What is the full forms of the following: D.N.A Deoxyribonucleic (The Free Dictionary, 2015) MRNA Messenger Ribonucleic Acid (The Free Dictionary, 2015) TRNA Transfer Ribonucleic Acid (The Free Dictionary, 2015) Q2. Complete the following sentences The synthesis of mRNA from DNA is known as:- Transcription (The Free Dictionary, 2015) The synthesis of proteins from RNA is known as:- Protein Synthesis (The Free Dictionary, 2015) Q3. Explain the process of Protein Synthesis detailing the role of DNA, mRNA, tRNA and ribosomes. The process where individual cells make protein is called protein synthesis. DNA and all RNA are both involved in this process. RNA are made by enzymes in the cell’s nucleus which start the process of protein synthesis by way of unwinding the wanted section of the DNA. A copy of one side of the DNA is formed by the RNA, and then is sent to all the other areas of the cell to help to bring altogether the different amino acids that make up proteins. As proteins are synthesised by mechanical and chemical processes within the cells, this process is called protein synthesis. Within the nucleus when the strand of RNA has been made, it is called messenger RNA MRNA. Through opening in the nucleus called pores the MRNA exits the nucleus and goes into the larger area of the cell which is called cytoplasm. As soon as the MRNA leaves the nucleus, it is attracted to a structure called ribosome, which are the cells work station or factory for protein synthesis. Within this point only one sub unit of ribosome is present. Another strand of RNA called transfer RNA, TRNA are activated as the MRNA start to attach to the ribosome sub unit. The TRNA stand finds the correct place to bind to the MRNA, as soon as it finds the place; it binds itself to the MRNA, which on one end holding amino acid. As this happens the other sub unit of ribosome come to completely form a complete structure. Another strand of TRNA appears as the ribosome encircles the strand of RNA. This strand is different from the first strand as it is carrying another amino acid; again the TRNA finds the correct place to bind to the MRNA. With the amino acid as soon as the second strand of TRNA is placed, both of the amino acid joins together aided by the ribosome, and adenosine triphosphate (ATP) with its cellular energy. As this pattern continues and repeats itself the chain of amino acid grows longer. As soon as all the amino acids have been placed in the correct sequence, a three-dimensional chain is made. O nce this has happened the protein is complete. When the proteins have been made the two sub-units of ribosome part to be joined later. In many ribosomes within the cell the process of protein synthesis take place there (Wisegeek, 2015). Q4.What is Point Mutation? Evaluate its effects. Point mutation is where there is a change within a gene, where one base pair in the DNA sequenced is changed or altered.Point mutation is most commonly the result of mistakes made during the DNA replication, however if DNA is exposed to x-rays and or ultraviolet radiations, this can also bring on point mutation. The two types of mutations are transition mutation and transversion mutation. Transition mutation happens when a pyrimidine base which is thymine (T) or cytosine (C) changes for another pyrimidine base, or it is when a purine base which is adenine (A) or guanine (G) changes for another purine base. Within a double stranded DNA each base is paired with a certain partner on the corresponding strand. A always pairs with T and C always pairs with G. For example a transition mutation is a GC base pair that swaps an AT base pair. However transversion mutation happens when a purine base changes for a pyrimidine base or the other way around i.e. when a TA or CG swaps for AT pair (Britannica, 2015). Point mutation can be put in three groups, nonsense mutation which is a code for a stop this can shorten the protein. Missense mutation which is a code for a different amino acid and silent mutation which is a code for the same or a different amino acid with no purposeful change in the protein itself. Missense mutation for example causes sickle cell disease, as the beta haemoglobin gene converts a GAG code into a GTG. This codes the amino acid valine instead of glutamic acid (Answers, 2015). The Effects of Point Mutation As all the cells within the body contain DNA therefore there are many places where point mutation can happen. However not all mutations are passed on to the offspring, these do not matter for evolution purposes. The mutations that matter for evolution purposes are those that can be passed on to the offspring, these happen in the reproductive cells eggs and sperm this type of mutation is called germ line mutation. There are three effects of germ line mutation:- No change happens in the phenotype, this is where there is no effect on the organism. This can happen in a part of the DNA strand where there is no function, or it can happen in a protein coding area but does not affect the amino acid sequence of the protein. Small changes happen in the phenotype, a single mutation can cause some cats ears to curl backwards. Significant changes happen in phenotype, this is where significant changes happen within the organism for example sickle cell disease. A single mutation can cause negative effects for the organism for example mutation that cause death, this is called lethal. Mutations are sometimes stereotyped as unimportant or the cause of genetic disease. While many mutations do have a negative effect, other mutations can have a small positive effect. Mutations that control genes can have a huge and sometimes positive effect. As some areas of the DNA control other genes, that decide when and where other genes are turned on, mutation in these parts can considerably change the way an organism is built. This can cause flow of effects in the behaviour of the genes under its control. Organisms have control genes that shape how the body is laid out i.e. the Hox gene is found in humans and animals. This gene sets out where the head goes and where limbs grow. These control gene build the bodies units i.e. limbs, head etc, therefore evolving a huge change in basic body lay out can be likely by a change in the Hox gene and natural selection (Understanding Evolution, 2015). Task 2 Q5. What is the probability of a couple having a boy or a girl? Male X Y Female X X XX = Girl XY= Boy The probability of a couple having a boy is 50%, the probability of a couple having a girl is 50%. Q6 What chromosomal abnormalities occur in the inheritance of Down’s syndrome Down syndrome is a result from trisomy 21, this is where the 21st chromosome has three chromosome and not the normal 2 chromosome. Therefore the individual will have 47 chromosomes and not the normal 46 chromosome. Down syndrome is cause by chromosomal abnormalities that happens randomly in the development stage of reproductive cells within a parent. This normally happens in the egg, but can sometimes happen in the sperm. The mistake in cell division results in a reproductive cell with abnormal number of chromosome, trisomy 21. This condition brings on intellectual disability, a characteristic facial appearance and weak muscle tone, all individual that are effected have cognitive delay (Genetics Home Reference, 2012). Q7. Explain inheritance of a sex-linked condition with a relevant example Hemophilia A and hemophilia B are both inherited sex linked traits, where they are inherited through the X linked recessive pattern. The genes that cause this condition are in the X chromosome, which is one half of the two sex chromosomes, the other being the Y chromosome. As males only have one X chromosome, one changed copy of the gene in the cell is enough to cause this condition. As females have two X chromosomes a mutation would have to have happened in both of the copies of the genes to cause this condition. This is very rare to happen in females as both copies of genes would have to have the same mutation happened to them. Therefore it is rare for females to have hemophilia. Fathers cannot pass on the inherited X linked condition to their sons as this is one of the characteristics of X linked inheritance. Females who have this X linked recessive condition have one mutated gene are called carriers. Female’s carriers have normally half the amount of coagulation factor whi ch is enough for blood clotting. Some female’s carriers have less than half of the normal amount of coagulation factor, these carriers are at risk for abnormal bleeding (About Education, 2015). Q8. Explain the inheritance of either cystic fibrosis or phenylketonuria Cystic fibrosis is a disorder that causes respiratory failure and vitamin deficiencies. Cystic fibrosis effects the secretion of the body i.e. saliva, mucus, sweat and digestive juices. As this secretion should be thin and watery, they are thick and sticky. This causes passageways to be clogged up within the body which causes damage to the pancreas and lungs. Cystic fibrosis is caused by inheriting defected gene from both parents, known as recessive gene. Cystic fibrous cannot be inherited by the off spring, if only one parent has the gene. If the off spring has only one defective gene then they are carriers of cystic fibrosis, this means that they will have the potential to pass on the defective gene to their off spring (eHow, 2015). The off spring labelled Ff are carriers, they have one defective gene but do not have the disorder themselves. In the diagram above both parents are carriers, they have one in four chances of producing an off spring that has cystic fibrosis (Bitesize, 2014). In the diagram above one parent is a carrier, while the other is not. They will not produce an off spring with cystic fibrosis, but are able to produce off springs that are carriers (Bitesize, 2014). Q9 Describe the process of genetic screening explaining the application of DNA recombinant technology in the treatment of a named medical condition.   Genetic testing is a medical test that can indicate any changes in the chromosomes, genes and proteins. The result of genetic testing can help to confirm or reject an alleged genetic condition, disease that the individual may have suspicion of, or help verify their chances of developing and or passing on a genetic disorder to their off spring. Some of the methods of genetic testing can be of these. Molecular genetic testing, this is where the medics study a single gene or short lengths of DNA to find defectiveness or mutation that may lead to a genetic disorder. Chromosomal genetic testing, this is where a whole chromosome and or long lengths of DNA to see if there are any sizable genetic changes i.e. extra copy of chromosome. Biochemical genetic testing, this is where the activity level or the amount of proteins are studied; any abnormalities that are detected can indicate genetic disorder (Genetic Home Reference, 2015). DNA recombinant technology, is a technology that produces DNA artificially. The DNA in living organism has been changed today due to this procedure. This procedure involves taking DNA from two difference sources and merging it together for it to become one single molecule. However this only works when the artificially created DNA has been reproduced, this is known as DNA cloning. The two types of cloning that DNA recombinant technology is used for are, therapeutic cloning and reproductive cloning. The reproductive cloning produces an organism with the exact same genetic information from the one that already is living. This type of cloning has been done with Dolly the sheep. Dolly was the first mammal to be reproduced as a genetic copy. Therapeutic cloning, reproduces tissues and or organ and not the whole organism. Therapeutic cloning has a great deal of benefit, i.e. if an organ has cancer it can be replaced with one made from the individuals own DNA. This reduces any likely hood of organ rejection within the body when a tissue or organ transplant is preformed (Wisegeek, 2015). Q10 Discuss the moral and ethical issues raised by DNA recombinant technology Not everyone is in favour of DNA recombinant technology, as they feel that science is playing god by reproducing unnaturally organisms that are not meant to be. And that science is devaluing the uniqueness of life itself. Also that some DNA work involves destroying embryos, this angers some social activist as this death is bought on intently this could be classed as murder. However scientist, argue that the purpose of DNA recombinant technology is to benefit and save human life, and not to destroy it (Wisegeek, 2015). References The free Dictionary 2015, DNA, [online] available at http://acronyms.thefreedictionary.com/DNA, Last Accessed 18/05/15 The free Dictionary 2015, MRNA, [online] available at http://acronyms.thefreedictionary.com/mRNA, Last Accessed 18/05/15 The free Dictionary 2015, TRNA, [online] available at http://acronyms.thefreedictionary.com/tRNA, Last Accessed 18/05/15 WiseGeek 2015, What is Protein Synthesis, [online] available at http://www.wisegeek.org/what-is-protein-synthesis.htm, Last accessed 19/05/15 Encyclopaedia Britannica 2015, Point Mutation, [online] available at http://www.britannica.com/EBchecked/topic/54744/point-mutation, Last accessed 19/05/15 Answers 2015, What is point mutation, [online] available at http://www.answers.com/Q/What_is_point_mutation, Last accessed 20/05/15 Understanding Evolution 2015, The effects of Mutation, [online] available at http://evolution.berkeley.edu/evolibrary/article/mutations_05, Last accessed 20/05/15 Genetics Home Reference 2012, Down Syndrome, [online] available at http://ghr.nlm.nih.gov/condition/down-syndrome, Last accessed 20/05/15 About Education 2015, Sex linked traits, [online] available at http://biology.about.com/od/genetics/ss/sex-linked-traits.htm, Last accessed 23/05/15 eHow 2015, How is Cystic Fibrosis Inherited, [online] available at http://www.ehow.com/how-does_5049940_cystic-fibrosis-inherited.html, Last accessed 23/05/15 Bitesize 2014, Cystic Fibrosis, [online] available at http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa_pre_2011/celldivision/inheritance4.shtml, Last accessed 23/05/15 Genetic Home Reference 2015, What is genetic testing, [online] available at http://ghr.nlm.nih.gov/handbook/testing/genetictesting, Last accessed 23/05/15 WiseGeek 2015, What is Recombinant DNA Technology, [online] available at http://www.wisegeek.org/what-is-recombinant-dna-technology.htm, Last accessed 23/05/15

Native American Oppression in North America Essay

While many different cultures were and are oppressed around the world, many people tend to forget about the genocide of the Native Americans on the land we call home. In 1492, when Christopher Columbus first sailed across the Atlantic Ocean, he came into contact with the indigenous people of the New World. After returning to Hispaniola, he quickly implemented policies of slavery and mass extermination of the Taino population in the Caribbean. This became the first major impact on Native Americans and eventually led to further oppression of American Indians. The implication of the population as savages helped in the displacement and genocide of the indigenous peoples. The Native Americans faced a lot of discrimination in North America during colonization, consisting of different forms of propaganda causing short-term and long-term effects in the present day. In 1492, a Spanish expedition headed by Christopher Columbus sailed for India to sell, buy, and trade rich spices and other goods, inadvertently discovering what is today North America. European conquest, large-scale exploration and colonization soon followed. This first occurred along the Caribbean coasts on the islands of Hispaniola, Puerto Rico and Cuba, and later extended into the interiors of both North and South America. Eventually, the entire Western Hemisphere came under the control of European governments, leading to profound changes to its landscape, population, and plant and animal life. From the 16th through the 19th centuries, the population of Indians declined from epidemic diseases brought from Europe, genocide and warfare at the hands of European explorers and colonists, displacement from their lands, internal warfare, enslavements, and a high rate of intermarriage. Epidemics of smallpox, typhus, influenza, diphtheria, and measles swept ahead of initial European contact, killing between 10 million and 20 million people, up to 95% of the indigenous population of the Americas. European expansion also caused many Native American tribes to lose their homes as they were forced by the government to live in certain areas called Indian Reservations. They were often poor and on the verge of starvation on these reservations. Many American Indians had to choose to assimilate to the culture of the colonists in order to live. The phrase â€Å"Kill the Indian, Save the Man† coincides with the assimilation. There were many tools to help with the assimilation of the natives such as boarding schools for Native American children, missionaries to introduce Christianity, and the strategic killing of their main food source, the bison. The Dawes Act was introduced in 1887 to get Native Americans to live like white Americans. Reservations were broken up into â€Å"allotments† that were given out to individual families and the families were supposed to farm and build homes on their allotment in order to support themselves. The plan failed due to the fact that some of the land was unsuitable for farming & ranching and some Natives refused to adopt a different way of life. Propaganda was a very powerful tool when it came to the oppression of American Indians. The term propaganda is derived from the Latin propagare, to propagate, to reproduce, to spread, with the meaning, to transmit, to spread from person to person. One form of early propaganda against Native Americans is the painting American Progress by John Gast in 1872. The painting depicts the iconographic image of Columbia, the American angel floating above the land, leading her pioneers westward. The angel image, intended as a personification of the United States, floats ethereally over the plains, stringing telegraph wire with one hand as she travels, and holding a schoolbook under her other arm. Ahead of her in the West is a great darkness populated by wild animals: bears, wolves, buffalo and Indian people. All are considered wild and savage, and fleeing away from her light. In her bright-light wake, as the figure progresses across the land, come farms, villages and homesteads and in the back are cities and railroads. The light of â€Å"civilization† dispels the darkness of â€Å"ignorance and barbarity†. American Indian people are portrayed along with the wild animals as the darkness, all of which have to be removed before Columbia can bring the prosperity promised to the United States. United States covert agencies working with the mainstream media often used â€Å"grey and black propaganda† to distort or fabricate information concerning the groups they had targeted. Grey propaganda efforts often centered upon contentions that the Indians’ main goal was to dispossess non-Indians of the home-owner, small farmer, or rancher type living within various treaty areas. For black propaganda there have been a number of highly publicized allegations of violence which, once disproven, were allowed to die without further fanfare. There were many short-term and long-term effects due to the oppression of American Indians. Many Native Americans were depicted as marauding, murdering, hellish savages who scalped women and children. They were seen as thieves, drunkards, and beggars, unwilling to work but willing to accept government handouts. The American Indian was often used as the antagonist in old country western films and portrayed in a negative, barbaric manner. Today a majority of the Native American population still resides on reservations. Despite helping shape America in their own way, the oppression of the American Indians is often overlooked in comparison to that of Jews during World War II and African Americans in the U. S.from slavery to the present-day Overall, the Native Americans overcame many things from when Columbus first came across them in the Caribbean in 1492. In the face of European exploration and colonization, genocide, epidemic diseases, and displacement among other things, American Indians managed to stay strong and hold on too as much of their culture as possible, working hard to dispel the false stereotype created by propaganda so long ago. While the discrimination of the past still affects them to this day, first nation peoples play a strong part in the development of this country.

Decision Making and Favorite Poem Essay

My absloute favorite poem we studied was â€Å"The stone† The way Wilfrid Wilson Gibson tells about the sorrow a woman goes through after the loss of her loved one is unmatched by anyone else. My third and final favorite poem was â€Å"Song† by Garcia Lorca. â€Å"Song† tells of â€Å"The girl of beautiful face† who â€Å"goes gathering olives†. The way Lorica paints a picture of content in the girl is what strikes me most about this poem. Many eligible men come by to take her away to their country. â€Å"Four ride’s†¦ on Andalusian ponies [say] come to Cordoba, lass†. But â€Å"the girl pays no heed†. Man after man comes to bring her home but she does not accept. This is because she is happy where she is, and she does not need anyone to make her feel content. I also liked the way the author uses personification, he writes â€Å"with gray arm of the wind encircling her waist†. Here he implies that the wind can grab the girls waist, when in fact that is not true. The author also uses free verse because there are no patterns in his writing. His writing sounds more like a paragraph then a poem to me. My second favorite poem was â€Å"Ordinance On lining Up† by Naomi Lazard. This is due to the message behind Lazard’s words. I believe that the author’s message in the poem is that people have to make many life altering changes in their life. Each decision, a metaphorical â€Å"path† they have to take, each have their perks and each have their downfalls. An example of this is â€Å"In joining the line to the right you ill end life as a beggar. If you decide on the line to the left everything you believe will become nonsense†. I also liked the use of metaphor in this poem. In fact, this whole poem is a metaphor. There are two lines, one on the right and one on the left. The author is saying you must make a decision, and comparing the decision to two lines. She uses free verse in her writing. This poem is written like a paragraph that has been cut at random places. There are sentences that end in the middle of lines, and there are sentences that go for more then two lines. The reason I liked this liked this poem is because I could relate to it closely. Every day I must make big decisions and small ones. Which ever way I choose, I will never know the outcome of the other one, but that is the risk we take getting out of bead in the morning. One of the biggest decisions I have had to make so far is whether go to go to Stuyvesant or go to the high school next to my house. They both had positives and negatives. Stuyvesant was farther away from my home, and is a much harder school that would require much more focus and work. The other school was ten minutes from my house and would not be as taxing on my brain. Although I could relate to this poem greatly, the message behind is was very obvious and did not require much thinking. Because of this it was only my second favorite poem out of the sixteen we studied. The poem which intrigued me the most was the first poem we studied, â€Å"The Stone† by Wilfrid Wilson Gibson. This poem was my favorite simply because of the use of language and visual imagery. An example of this is, â€Å"three days before, a splintered rock had struck her lover dead† this quote paints a picture of death in my mind that is unlike any other sentence I have read before. Another reason I loved this poem so much was the way Gibson shows you the lovers feeling of sorrow after the death of her loved one. The author states, â€Å"She did not sigh nor moan. His mother wept: She could not weep. Her lover slept: She could not sleep. Three days, three nights, She did not stir: Three days, three nights, Were one to her, Who never closed her eyes From sunset to sunrise, From dawn to evenfall, Her tearless, staring eyes,That, seeing naught, saw all. This shows how the heart reacts to devastating news. She was in such shock and sorrow that she could not even bring herself to cry. Among the other poetic devises used, I epically like the way the author uses personification, â€Å"The two of us were chiselling, Together, I and Death. † The author uses the word death so freely, it almost makes me feel like death is such a common thing. Through Gibsons use in language, visual imagery and overall feel of the poem, he has created a timeless masterpiece.

Psychic Passion - 947 Words

Psychic Passion: A Tale of Betrayal How can you trust someone you have never met face to face? By putting his faith in the psychic Andrew Morris, Detective Stephens sets himself up for failure in Sara Herrington’s Psychic passion, a novel of mystery, lust, and betrayal. Ultimately, Herrington’s book is about manipulation, and how easy it is to influence a trusting soul. Detective Stephens is a small town cop trying to make it in the big city of Birmingham, Alabama. He is haunted by his past and suffering accordingly. His wife left him, his kids hate him, and he struggles with a crippling urge to drink. To help cope with these urges, he phones Andrew Morris, who soothes him and feeds him generic psychic dribble: â€Å"†¦I see a change in†¦show more content†¦Adams tells Stephens that they have been watching Morris remove items from the scenes for days. He had been posing as a current detective and using Stephens name to get information. They were just wait ing for enough information to â€Å"make the arrest stick† (171). Everyone was in the know except Stephens. Andrew Morris was just an ex-detective who wanted to relive his glory days. Without his psychic wife, he needed a new way to get unwarranted attention. His way of doing that was to commit heinous crimes and use Stephens to get into the thick of things. Even after he is caught and exposed as a sick serial killer, he still attempt to manipulate Stephens into believing he’s insane, screaming from jail that his dead wife had framed him and that he was innocent. Stephens sees him for what he is at last, and realizes that serial killers all have something in common, â€Å"a [desire for a] sick thrill, and a vivid imagination† (179) Detective Stephens’s was a mistake any of us could make. When you are desperate, any voice of comfort can become a friend, someone you trust, and it doesn’t take someone of terrible cunning to manipulate you. 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